Nerve-like Polymer Network Created From Biological Tools

Sandia National Laboratories researchers using a succession of biological mechanisms, have created linkages of polymer nanotubes resembling the structure of a nerve, with many out-thrust filaments poised to gather or send electrical impulses.

Sandia National Laboratories researcher George Bachand said:

“This is the first demonstration of naturally occurring proteins assembling chemically created polymers into complex structures that modern machinery can’t duplicate.”

Sandia co-researcher Wally Paxton added:

“This is foundational science, but one possibility we see, way down the road, is to use soft artificial structures like these to painlessly interface with the body’s nerve structures.”

At present, rigid electrodes that cause inflammation are used to penetrate nerve tissue trying to communicate with an artificial limb, he explained. But in a future application, the polymer network could be used extend the nerve, providing a gentler prosthetic interface.

Enchanted Brooms

Creation of the neural structure, unachievable by normal manufacturing techniques, begins by altering the behavior of kinesin motor proteins—biological machines found in every human cell.

These tiny motors normally tote material from one part of a cell to another, carrying them on what, in video graphics, is portrayed as a vertical body with two legs. These stride along protein microtubules that form the cell structure.

The purposefulness of the motors resembles that of the spellbound brooms in Disney’s Fantasia, relentlessly carrying buckets of water up the castle stairs.

Turning natures’ machinery on its head, the researchers used known techniques to glue the “shoulders” of kinesin motors to a glass substrate. This prevents their bodies from travelling, but their “legs” above them continue their vigorous movements.

These pass microtubules above them, like an audience crowdsurfing entertainers on upraised hands.

Kinesin-powered Microtubules

In the next laboratory step, these travelling protein microtubules, microns in length, encounter relatively large polymer spheres, tens of microns in diameter, inserted by the researchers.

“At that point, we have structures that want to do work—the kinesin-powered microtubules—and something they want to do work on—the spheres,” Paxton said.

Sandia National Laboratories

Sandia National Laboratories researchers George Bachand and Wally Paxton at a confocal microscope illuminating the first biomolecular machines to assemble complex polymer structures. Credit: Randy Montoya

The microtubules, pre-coated with a sticky substance, nibble off polymer nanotubes from the sphere that lengthen as the kinesin motors travel on. The process resembles stringy strands of cheese lengthening as a piece of pizza is removed from a pan, said Paxton.

polymer nanotube netwoks

In these fluorescent microscopy images, the red image, left, shows only polymer nanotubes while the green image shows protein microtubules as well. Because polymer microspheres absorb more fluorescent dye than the nanotubes, the spheres are brighter. Each center “node” of the networks is about 20 microns in diameter, and some of the tubes are longer than 100 microns. Credit: Sandia National Laboratories

As the nanotubes lengthen and crosslink, they form structures complex enough to bring to mind the lights of a city seen at night from an airplane at high altitude. The networks range from hundreds of micrometers to tens of millimeters in total size and are composed of tubes 30 to 50 nanometers in diameter.

“One goal of our work is to make an artificial, highly branched neural structure,” said Bachand. “The next step is, can we wire them together? The answer is, the motors should do it naturally. And two such networks, joined together, would have self-healing built into them. The motors never stop running until they run out of fuel. A neural branch breaks, and then a motor can act on that area to produce a new branch.”

The insertion of quantum dots also turned out to be stable, which means that light could be used to carry information through the structure as well as electricity.

Reference:

Walter F. Paxton, Nathan F. Bouxsein, Ian M. Henderson, Andrew Gomez and George D. Bachand
Dynamic assembly of polymer nanotube networks via kinesin powered microtubule filaments
Nanoscale, 2015,7, 10998-11004 DOI: 10.1039/C5NR00826C

Top Illustration: Sandia National Laboratories